Deoxyribonucleic Acid Methyltransferase from the Eukaryote, Chlamydomonas reinhardi
نویسندگان
چکیده
منابع مشابه
Mitotic Replication of Deoxyribonucleic Acid in Chlamydomonas Reinhardi.
One of the central problems in molecular biology is to elucidate the replication mechanism of deoxyribonucleic acid (DNA) and its possible implication in genetic recombination. Of the several ingenious techniques that have been developed for the investigation of this problem,1' 2, the density gradient centrifugation technique developed by Meselson, Stahl, and Vinograd4 provides the most promisi...
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Cell-free extracts of the unicellular green alga, Chlnmydomonas reinhardi, catalyze the incorporation of acetyl-CoA and malonyl-CoA into long chain fatty acids. The fatty acid synthetase is dependent on added acyl carrier protein for activity, regardless if the cells are grown in the light or in the dark. The major products formed from acetyl-CoA and malonyl-CoA by cells in the light period of ...
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A new method has been developed for gene transfer in eukaryotic cells. Chlamydomonas reinhardi, a unicellular eukaryotic alga, was treated with a lethal dose of bleomycin, an agent that induces chromosome breakage. Bleomycin-treated cells were mated with untreated cells, and the mixture was plated onto selective agar medium. The progeny that arose contained the genetic markers from the untreate...
متن کاملDifferential activity of DNA methyltransferase in the life cycle of Chlamydomonas reinhardi.
Two molecular weight forms of DNA (cytosine-5-)-methyltransferase [S-adenosyl-L-methionine:DNA (cytosine-5-)- methyltransferase, EC 2.1.1.37], both active in assays in vitro, were isolated from the green alga Chlamydomonas reinhardi at various stages of the life cycle. The enzyme with Mr 60,000 was found in vegetative cells and gametes of both male (mt-) and female (mt+) mating types. The enzym...
متن کاملNutritional Control of Sexuality in Chlamydomonas Reinhardi
1. Cells of Chlamydomonas reinhardi grown in the light or dark on standard medium require an additional exposure to light in the absence of a nitrogen source, in order to become sexually active. As the culture ages, the light requirement decreases. 2. This light requirement is a function of nitrogen depletion, as shown by the observation that cells from cultures grown to maturity on a low nitro...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1980
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1980.tb04522.x